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Spectroscopy (pron.: //) is the study of the interaction between matter and radiated energy. Historically, spectroscopy originated through the study of visible light dispersed according to its wavelength, e.g., by a prism. Later the concept was expanded greatly to comprise any interaction with radiative energy as a function of its wavelength or frequency. Spectroscopic data is often represented by a spectrum, a plot of the response of interest as a function of wavelength or frequency.
Spectroscopy and spectrography are terms used to refer to the measurement of radiation intensity as a function of wavelength and are often used to describe experimental spectroscopic methods. Spectral measurement devices are referred to as spectrometers, spectrophotometers, spectrographs or spectral analyzers.
Daily observations of color can be related to spectroscopy. Neon lighting is a direct application of atomic spectroscopy. Neon and other noble gases have characteristic emission frequencies (colors). Neon lamps use collision of electrons with the gas to excite these emissions. Inks, dyes and paints include chemical compounds selected for their spectral characteristics in order to generate specific colors and hues. A commonly encountered molecular spectrum is that of nitrogen dioxide. Gaseous nitrogen dioxide has a characteristic red absorption feature, and this gives air polluted with nitrogen dioxide a reddish brown color. Rayleigh scattering is a spectroscopic scattering phenomenon that accounts for the color of the sky.
Spectroscopic studies were central to the development of quantum mechanics and included Max Planck's explanation of blackbody radiation, Albert Einstein's explanation of the photoelectric effect and Niels Bohr's explanation of atomic structure and spectra. Spectroscopy is used in physical and analytical chemistry because atoms and molecules have unique spectra. As a result, these spectra can be used to detect, identify and quantify information about the atoms and molecules. Spectroscopy is also used in astronomy and remote sensing on earth. Most research telescopes have spectrographs. The measured spectra are used to determine the chemical composition and physical properties of astronomical objects (such as their temperature and velocity).
One of the central concepts in spectroscopy is a resonance and its corresponding resonant frequency. Resonances were first characterized in mechanical systems such as pendulums. Mechanical systems that vibrate or oscillate will experience large amplitude oscillations when they are driven at their resonant frequency. A plot of amplitude vs. excitation frequency will have a peak centered at the resonance frequency. This plot is one type of spectrum, with the peak often referred to as a spectral line, and most spectral lines have a similar appearance.
In quantum mechanical systems, the analogous resonance is a coupling of two quantum mechanical stationary states of one system, such as an atom, via an oscillatory source of energy such as a photon. The coupling of the two states is strongest when the energy of the source matches the energy difference between the two states. The energy of a photon is related to its frequency by where is Planck's constant, and so a spectrum of the system response vs. photon frequency will peak at the resonant frequency or energy. Particles such as electrons and neutrons have a comparable relationship, the de Broglie relations, between their kinetic energy and their wavelength and frequency and therefore can also excite resonant interactions.
Spectra of atoms and molecules often consist of a series of spectral lines, each one representing a resonance between two different quantum states. The explanation of these series, and the spectral patterns associated with them, were one of the experimental enigmas that drove the development and acceptance of quantum mechanics. The hydrogen spectral series in particular was first successfully explained by the Rutherford-Bohr quantum model of the hydrogen atom. In some cases spectral lines are well separated and distinguishable, but spectral lines can also overlap and appear to be a single transition if the density of energy states is high enough.
Classification of methods 
Spectroscopy is a sufficiently broad field that many sub-disciplines exist, each with numerous implementations of specific spectroscopic techniques. The various implementations and techniques can be classified in several ways.
Type of radiative energy 
Types of spectroscopy are distinguished by the type of radiative energy involved in the interaction. In many applications, the spectrum is determined by measuring changes in the intensity or frequency of this energy. The types of radiative energy studied include:
- Electromagnetic radiation was the first source of energy used for spectroscopic studies. Techniques that employ electromagnetic radiation are typically classified by the wavelength region of the spectrum and include microwave, terahertz, infrared, near infrared, visible and ultraviolet, x-ray and gamma spectroscopy.
- Particles, due to their de Broglie wavelength, can also be a source of radiative energy and both electrons and neutrons are commonly used. For a particle, its kinetic energy determines its wavelength.
- Acoustic spectroscopy involves radiated pressure waves.
- Mechanical methods can be employed to impart radiating energy, similar to acoustic waves, to solid materials.
Nature of the interaction 
Types of spectroscopy can also be distinguished by the nature of the interaction between the energy and the material. These interactions include:
- Absorption occurs when energy from the radiative source is absorbed by the material. Absorption is often determined by measuring the fraction of energy transmitted through the material; absorption will decrease the transmitted portion.
- Emission indicates that radiative energy is released by the material. A material's blackbody spectrum is a spontaneous emission spectrum determined by its temperature. Emission can also be induced by other sources of energy such as flames or sparks or electromagnetic radiation in the case of fluorescence.
- Elastic scattering and reflection spectroscopy determine how incident radiation is reflected or scattered by a material. Crystallography employs the scattering of high energy radiation, such as x-rays and electrons, to examine the arrangement of atoms in proteins and solid crystals.
- Impedance spectroscopy studies the ability of a medium to impede or slow the transmittance of energy. For optical applications, this is characterized by the index of refraction.
- Inelastic scattering phenomena involve an exchange of energy between the radiation and the matter that shifts the wavelength of the scattered radiation. These include Raman and Compton scattering.
- Coherent or resonance spectroscopy are techniques where the radiative energy couples two quantum states of the material in a coherent interaction that is sustained by the radiating field. The coherence can be disrupted by other interactions, such as particle collisions and energy transfer, and so often require high intensity radiation to be sustained. Nuclear magnetic resonance (NMR) spectroscopy is a widely used resonance method and ultrafast laser methods are also now possible in the infrared and visible spectral regions.
Type of material 
Spectroscopic studies are designed so that the radiant energy interacts with specific types of matter.
Atomic spectroscopy was the first application of spectroscopy developed. Atomic absorption spectroscopy (AAS) and atomic emission spectroscopy (AES) involve visible and ultraviolet light. These absorptions and emissions, often referred to as atomic spectral lines, are due to electronic transitions of outer shell electrons as they rise and fall from one electron orbit to another. Atoms also have distinct x-ray spectra that are attributable to the excitation of inner shell electrons to excited states.
Atoms of different elements have distinct spectra and therefore atomic spectroscopy allows for the identification and quantitation of a sample's elemental composition. Robert Bunsen and Gustav Kirchhoff discovered new elements by observing their emission spectra. Atomic absorption lines are observed in the solar spectrum and referred to as Fraunhofer lines after their discoverer. A comprehensive explanation of the hydrogen spectrum was an early success of quantum mechanics and explained the Lamb shift observed in the hydrogen spectrum led to the development of quantum electrodynamics.
Modern implementations of atomic spectroscopy for studying visible and ultraviolet transitions include flame emission spectroscopy, inductively coupled plasma atomic emission spectroscopy, glow discharge spectroscopy, microwave induced plasma spectroscopy, and spark or arc emission spectroscopy. Techniques for studying x-ray spectra include X-ray spectroscopy and X-ray fluorescence (XRF).
The combination of atoms into molecules leads to the creation of unique types of energetic states and therefore unique spectra of the transitions between these states. Molecular spectra can be obtained due to electron spin states (electron paramagnetic resonance), molecular rotations, molecular vibration and electronic states. Rotations are collective motions of the atomic nuclei and typically lead to spectra in the microwave and millimeter-wave spectral regions; rotational spectroscopy and microwave spectroscopy are synonymous. Vibrations are relative motions of the atomic nuclei and are studied by both infrared and Raman spectroscopy. Electronic excitations are studied using visible and ultraviolet spectroscopy as well as fluorescence spectroscopy.
Crystals and extended materials 
The combination of atoms or molecules into crystals or other extended forms leads to the creation of additional energetic states. These states are numerous and therefore have a high density of states. This high density often makes the spectra weaker and less distinct, i.e., broader. For instance, blackbody radiation is due to the thermal motions of atoms and molecules within a material. Acoustic and mechanical responses are due to collective motions as well.
Pure crystals, though, can have distinct spectral transitions and the crystal arrangement also has an effect on the observed molecular spectra. The regular lattice structure of crystals also scatters x-rays, electrons or neutrons allowing for crystallographic studies.
Nuclei also have distinct energy states that are widely separated and lead to gamma ray spectra. Distinct nuclear spin states can have their energy separated by a magnetic field, and this allows for NMR spectroscopy.
Other types 
Other types of spectroscopy are distinguished by specific applications or implementations:
- Acoustic resonance spectroscopy is based on sound waves primarily in the audible and ultrasonic regions
- Auger spectroscopy is a method used to study surfaces of materials on a micro-scale. It is often used in connection with electron microscopy.
- Cavity ring down spectroscopy
- Circular Dichroism spectroscopy
- Coherent anti-Stokes Raman spectroscopy (CARS) is a recent technique that has high sensitivity and powerful applications for in vivo spectroscopy and imaging.
- Cold vapour atomic fluorescence spectroscopy
- Correlation spectroscopy encompasses several types of two-dimensional NMR spectroscopy.
- Deep-level transient spectroscopy measures concentration and analyzes parameters of electrically active defects in semiconducting materials
- Dual polarisation interferometry measures the real and imaginary components of the complex refractive index
- EPR spectroscopy
- Force spectroscopy
- Fourier transform spectroscopy is an efficient method for processing spectra data obtained using interferometers. Fourier transform infrared spectroscopy (FTIR) is a common implementation of infrared spectroscopy. NMR also employs Fourier transforms.
- Hadron spectroscopy studies the energy/mass spectrum of hadrons according to spin, parity, and other particle properties. Baryon spectroscopy and meson spectroscopy are both types of hadron spectroscopy.
- Hyperspectral imaging is a method to create a complete picture of the environment or various objects, each pixel containing a full visible, VNIR, NIR, or infrared spectrum.
- Inelastic electron tunneling spectroscopy (IETS) uses the changes in current due to inelastic electron-vibration interaction at specific energies that can also measure optically forbidden transitions.
- Inelastic neutron scattering is similar to Raman spectroscopy, but uses neutrons instead of photons.
- Laser-Induced Breakdown Spectroscopy (LIBS), also called Laser-induced plasma spectrometry (LIPS)
- Laser spectroscopy uses tunable lasers and other types of coherent emission sources, such as optical parametric oscillators, for selective excitation of atomic or molecular species.
- Mass spectroscopy is an historical term used to refer to mass spectrometry. Current recommendations are to use the latter term. Use of the term mass spectroscopy originated in the use of phosphor screens to detect ions.
- Mössbauer spectroscopy probes the properties of specific isotopic nuclei in different atomic environments by analyzing the resonant absorption of gamma-rays. See also Mössbauer effect.
- Neutron spin echo spectroscopy measures internal dynamics in proteins and other soft matter systems
- Photoacoustic spectroscopy measures the sound waves produced upon the absorption of radiation.
- Photoemission spectroscopy
- Photothermal spectroscopy measures heat evolved upon absorption of radiation.
- Pump-probe spectroscopy can use ultrafast laser pulses to measure reaction intermediates in the femtosecond timescale.
- Raman optical activity spectroscopy exploits Raman scattering and optical activity effects to reveal detailed information on chiral centers in molecules.
- Raman spectroscopy
- Saturated spectroscopy
- Scanning tunneling spectroscopy
- Time-resolved spectroscopy measures the decay rate(s) of excited states using various spectroscopic methods.
- Time-Stretch Spectroscopy
- Thermal infrared spectroscopy measures thermal radiation emitted from materials and surfaces and is used to determine the type of bonds present in a sample as well as their lattice environment. The techniques are widely used by organic chemists, mineralogists, and planetary scientists.
- Ultraviolet photoelectron spectroscopy (UPS)
- Vibrational circular dichroism spectroscopy
- X-ray photoelectron spectroscopy (XPS)
- Estimate weathered wood exposure times using near infrared spectroscopy.
- Cure monitoring of composites using Optical fibers
See also 
- Crouch, Stanley; Skoog, Douglas A. (2007). Principles of instrumental analysis. Australia: Thomson Brooks/Cole. ISBN 0-495-01201-7.
- Herrmann, R.; C. Onkelinx (1986). "Quantities and units in clinical chemistry: Nebulizer and flame properties in flame emission and absorption spectrometry (Recommendations 1986)". Pure and Applied Chemistry 58 (12): 1737–1742. doi:10.1351/pac198658121737.
- C.L. Evans and X.S. Xie.2008. Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine., doi:10.1146/annurev.anchem.1.031207.112754 Annual Review of Analytical Chemistry, 1: 883–909.
- W. Demtröder, Laser Spectroscopy, 3rd Ed. (Springer, 2003).
- F. J. Duarte (Ed.),Tunable Laser Applications, 2nd Ed. (CRC, 2009) Chapter 2.
- "Mass Spectrometry Terms and Definitions Project Page". Retrieved 2011-11-25.
- D. R. Solli, J. Chou, and B. Jalali, "Amplified wavelength–time transformation for real-time spectroscopy," Nature Photonics 2, 48-51, 2008. 
- J. Chou, D. Solli, and B. Jalali, "Real-time spectroscopy with subgigahertz resolution using amplified dispersive Fourier transformation," Applied Physics Letters 92, 111102, 2008. 
- "Using NIR Spectroscopy to Predict Weathered Wood Exposure Times".
- John M. Chalmers, and Peter Griffiths (Eds.), Handbook of Vibrational Spectroscopy (5 Volume Set), Wiley, New York (2002).
- Jerry Workman and Art Springsteen (Eds.), Applied Spectroscopy: A Compact Reference for Practitioners, Academic Press, Boston
- Spectroscopy links at the Open Directory Project
- Amateur spectroscopy links at the Open Directory Project
- MIT Spectroscopy Lab's History of Spectroscopy
- Timeline of Spectroscopy
- NIST government spectroscopy data